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. 2019 Jun 14;14(6):e0217724. doi: 10.1371/journal.pone.0217724

Table 1. NGS analysis of cultured human breast cancer MCF-7 cells.

DNAs extraction QC score Mean read depth Variant call VAF(%)  
Promega -0.004 472 GATA3p-355fs 50.6
PIK3CApGlu545Lys 53.4  
Beckman -0.001 620 GATA3p-355fs 48.7
PIK3CApGlu545Lys 45.1  
Qiagen -0.002 706 GATA3p-355fs 49.9
PIK3CApGlu545Lys 48.7  
Takara Bio -0.002 576 GATA3p-355fs 47.2
PIK3CApGlu545Lys 52.7  

VAF, Variant allele fraction; CNV, Copy number variation