Table 1. NGS analysis of cultured human breast cancer MCF-7 cells.
DNAs extraction | QC score | Mean read depth | Variant call | VAF(%) | |
---|---|---|---|---|---|
Promega | -0.004 | 472 | GATA3p-355fs | 50.6 | |
PIK3CApGlu545Lys | 53.4 | ||||
Beckman | -0.001 | 620 | GATA3p-355fs | 48.7 | |
PIK3CApGlu545Lys | 45.1 | ||||
Qiagen | -0.002 | 706 | GATA3p-355fs | 49.9 | |
PIK3CApGlu545Lys | 48.7 | ||||
Takara Bio | -0.002 | 576 | GATA3p-355fs | 47.2 | |
PIK3CApGlu545Lys | 52.7 |
VAF, Variant allele fraction; CNV, Copy number variation