Table 2. NGS analysis from cultured cancer cells.

cell	DNAs extraction	QC score	Mean read depth	Variant call	VAF(%)
Capan-1	Promega	-0.003	557	ARID1ApGln546*	58.6
				KRASpGly12Asp	81.0
				TP53pArg273His	100
	Beckman	-0.001	825	ARID1ApGln546*	53.4
				KRASpGly12Asp	81.0
				TP53pArg273His	99.8
	Qiagen	-0.002	854	ARID1ApGln546*	49.8
				KRASpGly12Asp	79.9
				TP53pArg273His	99.5
FTC133	Promega	-0.002	625	ERBB4pVal748Leu	99.4
				PTENpArg130*	99.8
				TP53pArg273His	100
				NF1pCys167*	99.4
				ERBB2pAla830Val	99.5
	Beckman	0.001	947	ERBB4pVal748Leu	100
				PTENpArg130*	99.8
				TP53pArg273His	99.6
				NF1pCys167*	99.0
				ERBB2pAla830Val	99.7
	Qiagen	0	882	ERBB4pVal748Leu	98.9
				PTENpArg130*	99.9
				TP53pArg273His	99.9
				NF1pCys167*	100
				ERBB2pAla830Val	99.8
MCF-7	Promega	-0.004	705	GATA3p-335fs	48.0
	Beckman	-0.001	824	GATA3p-335fs	52.2
	Qiagen	-0.002	950	GATA3p-335fs	50.6

VAF, Variant allele fraction

* indicates a generation of stop codon.