(A) Inflammation in Crohn’s disease (CD) patients correlates with higher Kaiso expression levels. (B)
KaisoTg mice display discontinuous lesions through the small intestine (red asterisks), blunted villi and thickening of the muscularis externa (black asterisks) compared to the NonTg siblings at 8-months of age. Insets are enlarged images of the areas outlined in yellow. (C) Line A (VKA) shows fewer crypt abscesses at 8 months of age compared to Line E (VKE) (n = 3 mice/phenotype). Statistical significance was determined by student’s t-test, and error bars are SEM *p <0.05. (D) Early age MPO analysis on age matched VKA and VKE mice show an increase in inflammation in VKE mice compared to VKA and NonTg mice at 14 weeks in SPF (n = 5–7 mice/genotype per age). Statistical significance was determined by two-way ANOVA, and error bars are SEM, ****p <0.0001. (E) At 14 weeks, VKE mice show a significant increase in neutrophils compared to age-matched NonTg mice and VKA mice. (n = 7–9 mice/genotype). Statistical significance was determined by one-way ANOVA, and error bars are SEM, *p <0.05.