Table 1.
Identity of genes differentially expressed between naive and experienced honeybees and in caffeine-treated honeybees
| Array data | ||||||
| bEST number | Gene | Head (foragers vs naive) | Abdomen (foragers vs naive) | Head (caffeine treated vs control) | Predicted function | Similarity to closest relative (%) |
| 5 | Hsc70 | -1.6 | +1.3 | +12.6 | Chaperone, heat shock response, neurotransmitter release | 95% |
| 22 | α-Gluc-1 | +2.5 | -1.1 | +2.2 | α-Glucosidase | 100% |
| 28 | Unknown | -3.2 | +2.3 | -1.5 | Unknown | N/A |
| 30 | Hsp20 | +1.3 | +2.1 | -1.1 | Chaperone | 65% |
| 31 | Hsp83 | -1.6 | +2.4 | +1.2 | Chaperone, heat shock response, protein folding | 92% |
| 36 | SPARC | -8.2 | -3.5 | +1.8 | Cell adhesion, calcium binding | 61% |
| 54 | GPDH 2 | -3.1 | +1.3 | -1.1 | Mitochondrial glycerol-3-phosphate dehydrogenase | 69% |
| 56 | Cox10 | -4.0 | -1.1 | -1.2 | Protoheme IX farnesyltransferase, cox assembly | 76% |
| 57 | Stck | -2.6 | -1.5 | +3.9 | LIM-domain protein, transcriptional regulation | 88% |
| 61 | Unknown | -5.2 | -1.3 | +1.4 | PDZ-domain protein, signal transduction | 50% |
| 82 | Aminopeptidase-1 | +1.6 | +1.0 | -1.2 | Membrane alanyl aminopeptidase | 51% |
| 92 | Trf | +1.2 | +3.0 | +2.8 | Iron transport, iron homeostasis, defense response | 63% |
| 97 | CG16857 | -3.3 | +2.1 | +1.5 | Ig-domain protein, cell adhesion | 63% |
| 99 | RpS19 | -4.7 | -1.7 | -1.6 | Structural protein of ribosome, protein biosynthesis | 83% |
| 102 | MRJP2 | +12.1 | +1.7 | -1.3 | Royal-jelly protein | 100% |
| 105 | Gld | +3.0 | -1.0 | +2.0 | Glucose dehydrogenase | 100% |
| 108 | PPIase | -4.0 | -1.1 | -1.5 | Peptidylprolyl isomerase | 92% |
| 109 | ATP synthase β | -5.9 | -1.4 | +1.6 | Hydrogen-transporting two-sector ATPase | 95% |
| 112 | Unknown | +5.5 | -1.1 | -1.3 | Unknown | N/A |
| 121 | IDGF | -1.4 | +1.9 | +2.4 | Imaginal disc growth factor, not chitinase | 54% |
| 122 | α-Glucosidase-2 | -2.3 | +9.8 | +1.2 | α-Glucosidase | 60% |
| 123 | Unknown | -3.3 | -1.1 | -1.7 | Unknown | N/A |
| 124 | Peritrophin | -5.6 | -4.3 | +1.4 | Structural protein of peritrophic membrane, chitin binding | 78% |
| 125 | Hymenoptaecin | +1.5 | +8.0 | +1.7 | Antibacterial protein, defense response | 100% |
| 127 | CG6112 | -7.1 | -3.8 | -1.3 | Ligand-gated ion channel subunit | 98% |
| 128 | Aminopeptidase-2 | -1.4 | -1.2 | +1.5 | Membrane alanyl aminopeptidase | 49% |
| 129 | Unknown | -1.9 | +1.2 | +5.6 | Unknown | N/A |
| 130 | Unknown | -7.2 | -1.7 | -1.1 | Unknown | N/A |
| 131 | Lectin | -16.0 | -5.9 | -1.3 | Ligand binding or carrier | 82% |
| 132 | CoxI | -4.9 | +1.0 | +1.1 | Respiratory-chain enzyme | 100% |
| 133 | Scp1 | -2.4 | -1.2 | -1.7 | Small chemosensory protein | 74% |
| 134 | ATPsynthase F0-6 | -14.1 | -1.4 | -1.0 | Proton pump, ATP synthesis | 100% |
| 136 | Unknown | N/A | +1.2 | +4.3 | Unknown | N/A |
| 138 | Gs2 | N/A | +1.8 | +3.5 | Glutamine synthase | 77% |
| 143 | Unknown | N/A | -1.0 | +3.4 | Unknown | N/A |
| 147 | CG5586 | N/A | -1.3 | -3.2 | WD40 and SOCS domain-containing protein | 38% |
| 148 | Aminotransferase | -2.6 | -1.4 | -3.5 | Ornithine-oxo-acid aminotransferase | 73% |
bEST number, cDNA number; gene, gene identifier; array data, fold change calculated from array hybridization data using pairwise comparisons for heads, abdomens and caffeine-treated bees; predicted function, putative function inferred from sequence similarity; Percentage similarity to the closest relative in GenBank. Accession numbers of bESTs reported in this paper: 5, BI946410; 22, BI946425; 28, BI946431; 30, BI946433; 31, BI946435; 36, BI946440; 54, BI946454; 56, BI946456; 57, BI946458; 61, BI946461; 82, BI946480; 92, BI946487; 97, BI946490; 99, BI946493; mrjp2 (102), af000632; gld (105), ab022907; 108, BI946499; 109, BI946500; 112, BI946503; 121, BI946511; 122, BI946512; 123, BI946513; 124, BI946514; hymenoptaecin (125), amu15956; 127, BI946517; 128, BI946519; 129, BI946520; 130, BI946522; 131, BI946524; 132, BI946525; 133, BI946526; 134, BI946527; 136, BI946528; 138, BI946532; 143, BI946537; 147, BI946542; 148, BI946543.