Fig 7. Effects of cordycepin and deoxyformycin on host cell and intracellular Mycobacterium bovis BCG.

(A) Determination of LDH cytotoxicity of cordycepin or deoxyformycin in BMDMs. BMDMs (5×10⁴ cells per well) were plated in a 96-well plate and incubated overnight in an incubator at 37°C, 5% CO₂. And on the next day, different concentrations of cordycepin or deoxyformycin were added to the culture media and incubated for 18 hours. LDH cytotoxicity was measured using the Pierce LDH Cytotoxicity Assay Kit. All experiments were repeated three times. Error bars are standard deviations. (B) BMDMs were infected with Mycobacterium bovis BCG (MOI = 1) and treated with 0.4 mM cordycepin or/and 0.4 mM deoxyformycin for 18 h, and the mycobacterial survival was assayed by determining colony-forming units (CFUs). All experiments were repeated three times. Error bars represent the SD in the figure. Asterisks indicate P-values (*, P<0.05; ***, P<0.001) from the unpaired two-tailed Student’s t-test using GraphPad Prism 5.