Table 3. Phenotypic properties of B. subtilis strains.
| Genotype | Doubling timea (min) | Sporulation efficiencyb (%) | DPA releasec (μg/ml/OD) | NAM releasec (nmole/ml/OD) | % phase-dark sporesd |
|---|---|---|---|---|---|
| Wild type | 20 | 66 | 5.3 ± 0.1 | 61.0 ± 14.2 | 95 |
| dnaJ | 31 | 89 | 1.1 ± 0.4* | 25.9 ± 12.7 | 14 |
| hfq | 40 | 63 | 2.2 ± .03* | 30.1 ± 6.2 | 48 |
| pcrB | 31 | 68 | 3.0 ± 0 | 30.3 ± 8.0 | 41 |
| phoP | 44 | 83 | 2.7 ± 0.4* | 46.6 ± 5.8 | 63 |
| phoR | 35 | 54 | 3.9 ± 0.5 | 39.2 ± 9.5 | 86 |
| sipT | 34 | 54 | 2.3 ± 1.0* | 23.5 ± 10.2* | 22 |
| skfE | 27 | 59 | 1.8 ± 0* | 40.6 ± 10.7 | 38 |
| ygaC | 21 | 71 | 3.1 ± 0.4* | 36.6 ± 9.9 | 60 |
| ylbC | 29 | 48 | 1.1 ± 0.3* | 14.3 ± 3.4* | 19 |
| yqeF | 23 | 84 | 1.9 ± 0* | 37.2 ± 9.4 | 50 |
| yqhL | 20 | 53 | 2.6 ± 0.4* | 36.1 ± 8.1 | 69 |
| ytpA | 22 | 95 | 1.63 ± 0* | 17.7 ± 2.7* | 55 |
| ytxG | 31 | 18 | 4.8 ± 0.3 | 50.9 ± 4.8 | 90 |
| yybT | 21 | 69 | 4.7 ± 0.4 | 56.6 ± 14.1 | 92 |
a Growth in 2xSG medium at 37°C
b Heat-resistant count/total viable count after 24 hr incubation on 2xSG medium at 37°C.
c Release of DPA and NAM 30 or 45 min, respectively, after exposure to 10 mM L-Val at 37°C.
* indicates a significant difference from the wild type (T-test, p<0.05). Values are indicative of averages and standard deviations of three biological replicates.
d Spores pixel intensities quantified and classified as described in Materials and Methods after 60 min exposure to 10 mM L-Val at 37°C.