Table 2. Primer sequences for gene expression analysis by qPCR.
| Gene | Accession No | Primers | Annealing Temperature (°C) | Amplicon (bp) |
|---|---|---|---|---|
| HvVIP1 | AK365082 |
5’-GAGAGCTCAAACTCCGGTTG -3’ 5’-GAACTTGACCTGCCGCTATC -3’ |
60 | 114 |
| HvPR1 | X74939 |
5’-CGCCGGGAATGTTGTTGGAC-3’ 5’-ACGAGCGTGCATGAATCCCA-3’ |
60 | 126 |
| HvPR3 | X78672 | 5’-AAGGCCACGTCTCCACCCTA-3’ 5’CAGGTCCGGGTTGCTCACAA-3’ | 58 | 114 |
| HvPR4 | Y10814 |
5’-GCCAAGATCGACACCAACGG-3’ 5’-CAGTCCGAGCTGCTCACTCA-3’ |
60 | 150 |
| HvPR5 | AJ276225 |
5’-ACGACATCTCGGTTATCGACG-3’ 5’-TTATTGCCACTGCAGGCGT-3’ |
60 | 151 |
| HvPR10 | AY220734 |
5’-GCGGCTAGGGTGTTCAAGAC-3’ 5’-CCTGAGCTTCGCCACACAAG-3’ |
60 | 123 |
| HvMPK1 | AK376245 |
5’ -GGTCACCGCCAAGTACAA -3’ 5’ -AAGGCGTTGGCGATCTT -3’ |
59 | 123 |
| HvACT | AK362208 |
5’-CGTGTTGGATTCTGGTGATG -3’ 5’-AGCCAC ATATGCGAGCTTCT -3’ |
60 | 208 |