| Antibodies |
| Mouse monoclonal anti-FLAG (clone M2) |
Sigma |
Cat# F1804, Lot SLBS353OV (RRID:AB_262044) |
| Rabbit monoclonal anti H3K27me3 (clone C36B11) |
Cell Signaling Technology |
Cat# 9733, Lot 8 (RRID:AB_2616029) |
| Rabbit monoclonal anti-PDGFRα (clone D13C6) |
Cell Signaling Technology |
Cat# 5241, Lot 3 (RRID:AB_10692773) |
| Rabbit polyclonal anti-Olig2 |
Millipore |
Cat# AB9610, Lot 2060464 (RRID:AB_570666) |
| Rabbit monoclonal anti-H3K27ac (clone D5E4) |
Cell Signaling Technology |
Cat# 8173, Lot 1 (RRID:AB_10949887) |
| Rabbit monoclonal anti-H3K4me3 (clone C42D8) |
Cell Signaling Technology |
Cat# 9751, Lot 8 (RRID:AB_2616028) |
| Normal rabbit IgG |
Cell Signaling Technology |
Cat# 2729S (RRID:AB_1031062) |
| Biotinylated Horse anti-mouse IgG |
Vector Laboratories |
Cat# BA-2000, (RRID:AB_2313581) |
| Biotinylated Goat anti-rabbit IgG |
Vector Laboratories |
Cat# BA-1000 (RRID:AB_2313606) |
| Rabbit polyclonal anti-H3.3 K27M |
Millipore |
Cat# ABE419 (RRID:AB_2728728) |
| Rabbit polyclonal anti-H3 |
Abcam |
Cat# ab1791, Lot GR153323–6 (RRID:AB_302613) |
| HRP-conjugated Donkey anti-rabbit IgG |
GE Healthcare Life Sciences |
Cat# NA934, Lot 9495175 (RRID:AB_772206) |
| IRDye 800CW Goat anti rabbit IgG |
LI-COR |
Cat# 926–32213, Lot C70918–03 (RRID:AB_621848) |
| Biological Samples |
| Human primary DIPG tumor samples |
(Wu et al., 2014) |
NA |
| Chemicals, Peptides, and Recombinant Proteins |
| Geneticin (G418) |
Life Technologies |
Cat# 10131–027 |
| Puromycin |
Sigma |
Cat# P8833 |
| AlexaFluor 488–5-dUTP |
ThermoFisher |
Cat# C11397
|
| AlexaFluor 594–5-dUTP |
ThermoFisher |
Cat# C11400
|
| Mouse NeuroCult Proliferation Medium |
Stem Cell Technologies |
Cat# 05702 |
| Human EGF |
Miltenyi Biotech |
Cat# 130–097-751 |
| Accutase |
Millipore |
Cat# SCR005 |
| Methyl cellulose |
Sigma |
Cat# M0512 |
| SYTO 9 Green Fluorescent Nucleic Acid Stain |
ThermoFisher |
Cat# S34854
|
| Tamoxifen |
Sigma |
Cat# T5648 |
| Corn oil |
Sigma |
Cat# C8267 |
| Hematoxylin |
ThermoFisher |
Cat# 7221 |
| Eosin |
ThermoFisher |
Cat# 7111 |
| Trizol |
Invitrogen |
Cat# 15596–018 |
| ERCC RNA Spike-In Mix |
Life Technologies |
Cat# 4456740, Lot 1412017 |
| Protease inhibitors |
Sigma |
Cat# P8340 |
| Protein A sepharose beads |
GE Healthcare Life Sciences |
Cat# 17–5280-04 |
| H3K27me3 peptide |
AnaSpec |
Cat# AS-64378, Lot 1457617 |
| Quantitect SYBR Master Mix |
Qiagen |
Cat# 204145 |
| SuperSignal West Dura Extended Duration Substrate |
ThermoFisher |
Cat# 34076 |
| Critical Commercial Assays |
| QuickChange II SDM Kit |
Agilent Technologies |
Cat# 200523 |
| Vectastain Universal Elite ABC Kit |
Vector Laboratories |
Cat# PK-6100 |
| DAB Substrate Kit |
Vector Laboratories |
Cat# SK-4100 |
| SuperScript III First-Strand Synthesis System |
ThermoFisher |
Cat# 18080051 |
| QIAquick PCR Purification Kit |
Qiagen |
Cat# 28104 |
| Deposited Data |
| RNA sequencing mapped raw data |
This paper |
GEO: GSE108364
|
| ChIP sequencing mapped raw data |
This paper |
GEO: GSE108364
|
| Experimental Models: Cell Lines |
|
Drosophila melanogaster D.MEL S2 |
ATCC |
CRL-1963 (RRID:CVCL_Z232) |
| Experimental Models: Mouse Strains |
| B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J |
The Jackson Laboratory |
Stock# 012930 (RRID:IMSR_JAX:012930) |
| C57BL/6J |
The Jackson Laboratory |
Stock# 000664 (RRID:IMSR_JAX:000664) |
|
H3f3aLSL-K27M-Tag
|
This paper |
NA |
|
H3f3aLSL-WT-Tag
|
This paper |
NA |
| Nestin-Cre |
The Jackson Laboratory (Tronche et al., 1999) |
Stock# 003771 (RRID:IMSR_JAX:003771) |
|
LSL-PDGFRAV544ins
|
This paper |
NA |
|
Nestin-CreERT2
|
(Zhu et al., 2012) |
NA |
|
Trp53flox
|
The Jackson Laboratory (Jonkers et al., 2001) |
Stock# 008462 (RRID:IMSR_JAX:008462) |
| Oligonucleotides |
|
LSL-PDGFRAV544ins Forward |
Integrated DNA Technologies |
5’-TCTGCTAACCATGTTCATGCCTTC-3’ |
|
LSL-PDGFRAV544ins Reverse |
Integrated DNA Technologies |
5’-GACCGAAAGGAGCGCACGAC-3’ |
|
Pbx3 Forward |
Integrated DNA Technologies |
5’-CCTCTAGAGAACTTGGCGCT-3’ |
|
Pbx3 Reverse |
Integrated DNA Technologies |
5’-GGAAGTGCAACTTTCTCCGC-3’ |
|
Eya1 Forward |
Integrated DNA Technologies |
5’-CCTGCACACTCGCTACCT-3’ |
|
Eya1 Reverse |
Integrated DNA Technologies |
5’-CTCAGATGCTATCTGCCGCT-3’ |
|
Meis2 Forward |
Integrated DNA Technologies |
5’-AGCCGAGACTTCTGAGTTGT-3’ |
|
Meis2 Reverse |
Integrated DNA Technologies |
5’-AGTGGGGATCGTTGTTGGTA-3’ |
| Recombinant DNA |
| Plasmid: Lox-STOP-lox (LSL) |
Addgene |
Addgene Plasmid #11584 |
| Software and Algorithms |
| Gene Ontology Consortium |
(The Gene Ontology, 2017) |
http://www.geneontology.org |
| Enrichr |
(Kuleshov et al., 2016) |
http://amp.pharm.mssm.edu/Enrichr/ |
| Samtools v0.1.18 |
(Li et al., 2009) |
https://github.com/samtools/samtools |
| BEDtools v2.25.0 |
(Quinlan and Hall, 2010) |
http://bedtools.readthedocs.io/ |
| UCSC tools |
NA |
https://genome.ucsc.edu/util.html |
| BWA v0.7.12 |
(Li and Durbin, 2010) |
http://bio-bwa.sourceforge.net |
| R |
NA |
https://www.r-project.org |
| Bioconductor |
NA |
https://www.bioconductor.org/ |
| IGV v2.3.82 |
(Thorvaldsdottir et al., 2013) |
http://software.broadinstitute.org/software/igv/ |
| HOMER v4.8.3 |
(Heinz et al., 2010) |
http://homer.ucsd.edu/ |
| HTSeq v0.6.1p1 |
(Anders et al., 2015) |
https://htseq.readthedocs.io/ |
| MACS2 V2.1.1 |
(Zhang et al., 2008) |
https://github.com/taoliu/MACS |
| SICER v1.1 |
(Zang et al., 2009) |
https://home.gwu.edu/~wpeng/Software.htm |
| FactoMineR |
(Le et al., 2008) |
https://CRAN.R-project.org/package=FactoMineR |
| GSEA |
(Subramanian et al., 2005) |
http://software.broadinstitute.org/gsea/index.jsp |
| Prism 7.0d |
GraphPad |
NA |
| PrimerBLAST |
NCBI |
https://www.ncbi.nlm.nih.gov/tools/primer-blast/ |
| Other |
| Ultra-low attachment T25 cell culture flasks |
Corning |
Cat# 3815 |
| 96-well, black walled cell culture plates |
Corning |
Cat# 3904 |
| 30-gauge insulin syringe |
Becton Dickenson |
Cat# 309301 |
| 0.45μm nitrocellulose membrane |
GE Healthcare Life Sciences |
Cat# RPN2020D |
