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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Immunol. 2019 Mar 27;202(9):2585–2608. doi: 10.4049/jimmunol.1801350

Fig. 2. Immunophenotyping of activated T cells in decidual tissues.

Fig. 2.

(A) Gating strategy used to identify CD4+ and CD8+ T cells expressing granzyme B or perforin in the decidua basalis from women who delivered at term without labor (TNL), term with labor (TIL), preterm without labor (PTNL), or preterm with labor (PTL). (B) Proportions of CD4+granzyme B+ or CD8+granzyme B+ T cells. (C) Proportions of CD4+perforin+ or CD8+perforin+ T cells. The p-values were determined by 2-tailed Mann-Whitney U-test. Data are shown as scatter plots (median). Demographic and clinical characteristics of the study population are shown in Table II. (D) qPCR ratio of SRY (Y-chromosome) to AR (X-chromosome) expression in CD3+ T cells isolated from umbilical cord blood, maternal peripheral blood, the decidua basalis, and the decidua parietalis. Data are shown as scatter plots (median + interquartile range). Gel image of amplified PCR fragments shows SRY expression by CD3+ T cells isolated from umbilical cord blood, maternal peripheral blood, the decidua basalis, and the decidua parietalis. HBB = human β-globin (housekeeping gene).