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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Immunol. 2019 Mar 27;202(9):2585–2608. doi: 10.4049/jimmunol.1801350

Fig. 4. Activation of T and B cells at the maternal-fetal interface and myometrium prior to preterm birth.

Fig. 4.

(A) Gating strategy used to identify activated CD4+ (CD3+CD4+ cells expressing CD25, CD69, or IL2) and CD8+ (CD3+CD8+ cells expressing CD25, CD69, or IFNγ) T cells at the maternal-fetal interface. Grey histograms represent autofluorescence controls, and colored histograms represent the expression of the CD3 molecule as well as CD25, CD69, IL2, or IFNγ. (B) Mean fluorescence intensity (MFI) of the CD3 molecule in decidual and myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486 or DMSO (n=8-13 each). Proportions of (C) CD4+ and (D) CD8+ T cells expressing CD25 in decidual and myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486, or DMSO (n=8 each). Proportions of (E) CD4+ and (F) CD8+ T cells expressing CD69 in decidual and myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486, or DMSO (n=8 each). (G) Proportion of CD4+ T cells expressing IL2 in decidual and myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486, or DMSO (n=8-13 each). (H) Proportion of CD8+ T cells expressing IFNγ in decidual and myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486 or DMSO (n=8-13). (I) Gating strategy used to identify activated B cells (CD45+CD19+IFNγ+) at the maternal-fetal interface. The grey contour plot represents the autofluorescence control and the colored contour plot represents the expression of IFNγ. (J) Proportion of B cells expressing IFNγ in decidual or myometrial tissues from dams injected with αCD3ε, isotype, LPS, PBS, RU486 or DMSO (n=9-13 each). The p-values were determined by 2-tailed Mann-Whitney U-test. Data are shown as scatter plots (median).