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(A) The lordosis phenotype (red arrow) caused by cnot3a knockdown was rescued by co-injection of 250 pg wildtype human CNOT3 (hCNOT3) with 4 ng cnot3a morpholino (mo). (B) Percentage of rescue after co-injection of hCNOT3, E20K, or E70K variants with cnot3a mo (n > 75 per group). Significance of co-injection rescue was determined using Fisher’s exact test compared to cnot3a mo injection alone. ns = not significant. ****= p-value < 0.0001.
