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. 2019 Jun 11;10(39):3939–3951. doi: 10.18632/oncotarget.27003

Figure 4. Human CNOT3 rescues intestinal defects in zebrafish apcmcr embryos.

Figure 4

(A) Graph shows cnot3a mRNA expression relative to 18s in 72 hpf embryos. (B) apcmcr and wildtype embryos, WISH staining for gut marker fabp2 for vehicle and hCNOT3 injected apcmcr embryos. (C) WISH staining for hCNOT3 E20K and E70K-injected apcmcr embryos and vehicle-injected apcWT. Note that the red arrowhead = fabp2 staining. (D) Percentage of apcmcr fish with gut marker staining from (A and B) at 72 hpf (n > 10 per group). Statistical significance of CNOT3 mRNA injection rescue was determined using Fisher’s exact test compared to uninjected apcmcr embryos alone. ns = not significant; *** = p-value = 0.0003; ** = p-value = 0.0043.