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. 2019 May 22;8:e46054. doi: 10.7554/eLife.46054

Figure 7. Model depicting the early stages of de novo centriole formation.

Figure 7.

A PLK4 focus forms and phosphorylates STIL at S1116 in the STAN motif to recruit SAS6 and initiate cartwheel assembly. Phosphorylation of STIL at S428 by PLK4 promotes CPAP binding and connects the growing cartwheel to the triplet microtubules of the centriole wall. In the absence of STIL S428 phosphorylation, STIL is able to recruit SAS6 and promote cartwheel assembly. However, STIL S428A fails to recruit CPAP and link the cartwheel to the microtubule wall, leading to a failure of de novo centriole assembly. In the absence of STIL S1116 phosphorylation, STIL is not effectively recruited to a PLK4 focus and cartwheel assembly fails.