Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapien) | DLD-1 LacZeo | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | available upon request | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL WT |
Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | available upon request | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL S1116A | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | available upon request | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL S428A | this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL PRP | this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL PRP/28A | this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL 28A/16A | this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL PRP/16A | this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-STIL PRP/28A/16A |
this study | DLD-1 cell line in which a Myc-GFP-STIL construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-CPAP WT | this study | DLD-1 cell line in which a Myc-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-CPAP F1229A | this study | DLD-1 cell line in which a Myc-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-GFP-CPAP E1235K | this study | DLD-1 cell line in which a Myc-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-STIL-T2A-GFP-CPAP | this study | DLD-1 cell line in which a Myc-STIL-T2A-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-STIL S428A -T2A-GFP-CPAP | this study | DLD-1 cell line in which a Myc-STIL-T2A-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-STIL PRP -T2A-GFP-CPAP | this study | DLD-1 cell line in which a Myc-STIL-T2A-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Cell line (H. sapien) | DLD-1 LacZeo; Myc-STIL S1116A -T2A-GFP-CPAP | this study | DLD-1 cell line in which a Myc-STIL-T2A-GFP-CPAP construct with indicated mutation is integrated at a single locus | |
| Antibody | Mouse monoclonal, Myc | EMD Millipore | Cat. No. 05–724 | (WB) use 1:1000 |
| Antibody | Mouse monoclonal, Flag | Sigma | Cat. No. F1804 | (WB) use 1:1000 |
| Antibody | Rabbit polyclonal, mCherry | gift from Joo Soek-Han | (WB) use 1:1000 | |
| Antibody | Rabbit polyclonal, STIL (WB) | Bethyl | Cat. No. A302-441A | (WB) use 1:2500 |
| Antibody | Rabbit polyclonal, STIL pS428 | this study | see Materials and methods. (WB) use 1:1000 | |
| Antibody | Rabbit polyclonal, STIL pS1116 | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | (WB) use 1:1000 | |
| Antibody | Rabbit polyclonal, STIL pS1108 | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | (IF) use 1:250 | |
| Antibody | Mouse monoclonal, tubulin | Sigma | Cat. No. T6199 | (WB) use 1:1000 |
| Antibody | Rabbit polyclonal, Plk4 pT170 | this study | see Materials and methods. (WB) use 1:1000 | |
| Antibody | Goat polyclonal, CEP192 | this study | see Materials and methods. (IF) use 1:1000 | |
| Antibody | Rabbit polyclonal, Centrin | this study | see Materials and methods. (IF) use 1:1000 in | |
| Antibody | Rabbit polyclonal, CPAP | gift from Karen Oegema | (WB or IF) use 1:1000 | |
| Antibody | Rabbit polyclonal, STIL (IF) | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | (IF) use 1:250 | |
| Antibody | Rabbit polyclonal, Plk4 | Moyer et al., 2015. DOI: 10.1083/jcb.201502088 | (IF) use 1:1000 | |
| Antibody | Mouse monoclonal, SAS6 | Santa Cruz | Cat. No. sc-81431 | (IF) use 1:200 |
| Chemical compound, drug | centrinone | Tocris Bioscience | Cat. No. 5687 | use at 500 nM |