Fig. 3. Evaluation of PTMAO immunogenicity.
C57BL/6J mice were subcutaneously injected with either PEG-KLH or PTMAO-KLH (n = 5) for five doses (one dose per week). SC, subcutaneous. Mice blood was collected on day 7, 14, 21, 28, and 35 (A). The titer of polymer-specific IgM (B) and IgG (C) in mice sera was detected with ELISA tests. All statistical analyses were performed using Student’s t test. NS, no significant difference. ***P < 0.001.