IP using the TAP-tag is detected by Western Blot analysis with tag specific antibody to show the IP quality of the different PAR-CLIP experiments representative for one replicate per factor. The factors are sorted according to their complexes: deadenylation (Ccr4, Pop2, Not1, Caf40, Pan3, and Pan3), decapping (Dcp1, Dcp2, Edc2, Edc3, and Dhh1), 5´→3´ exonuclease (Xrn1), exosome (Rrp6, Csl4, Rrp40, Rrp4, and Rrp44), TRAMP (Air1, Trf5, Mtr4, Air2, and Trf4), Ski (Ski2, Ski3, Ski7, and Ski8), and NMD (Upf1, Upf2, Upf3, and Nmd4). The molecular weight including the weight of the TAP tag (in kDa) is indicated for each factor. The band at ~25 kDa is caused by cross-reactivity of the light chain of the used antibodies for IP and Western Blot. A shift to higher molecular weight than indicated can be caused by UV-crosslinking of proteins to RNA.