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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Feb 25;120(8):12450–12460. doi: 10.1002/jcb.28512

Figure 2. Bone marrow stromal cells contribute to inhibitory effect of M-CSF on bone marrow cell OC differentiation.

Figure 2.

A). Representative FACS analysis shows that M-CSF pretreatment decreased the percentage of c-Kit-CD11b+ cell in 1adBMC. B) Quantification of the percentage of c-Kit−CD11b+ from 1adBMC treated with or without 10ng/ml M-CSF for 24 h. C). FACS analysis showing the purity of APC-c-Kit+ and PE-CD11b+ magnetic bead-purified cells. D). MTT assay shows M-CSF stimulated c-Kit+ and c-Kit-CD11b+ cell proliferation. E and F). Representative TRAP staining images from c-Kit+ or c-Kit-CD11b+ cells subjected to M-CSF pretreatment and then OC differentiation. G). Quantification of multinuclear TRAP+ cells from E, F. Note: *: p<0.05; ***: p<0.001.