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Effects of OBs on levels of 19S-DUBs and PARP in breast cancer MDA-MB-468 (A) and MCF-7 (B) cells. MDA-MB-468 and MCF-7 cell lines were treated with PBS or 15 μM of DAST, FB-28 and FB-71 for 12 hours. The cells were harvested, and protein lysates were extracted for electrophoresis, immunoblotting for UCHL5 (a), USP14 (b) and PARP (c) as well as β-actin (d; as an internal control for protein loading and normalization between samples).
