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. Author manuscript; available in PMC: 2020 Jun 13.
Published in final edited form as: Cell. 2019 May 23;177(7):1915–1932.e16. doi: 10.1016/j.cell.2019.04.040

Figure 2. Four subsets of HSC regulator-producing Lepr-MSCs form a differentiation continuum.

Figure 2.

(A–C) Signature genes for Lepr-MSCs in the stroma atlas. (A,B) tSNE of Figure 1B colored by cluster-1 membership (A), or by expression (color bar, TP10K) of key MSC marker genes (B, left) (right), along with the corresponding distributions of expression levels (ln(TP10K+1), y axis) across clusters of Figure 1B (x axis). (C) Expression (row-wide z-score of ln of average TP10K) of top differentially expressed genes (rows) in the cells of each cluster (columns) (color bar, top, as in Figure 1B), ordered by five gene categories (labels on left). (D–F) Four Lepr-MSC subclusters span a differentiation continuum. (D,E) Continuous transition across the subclusters. Four subclusters colored on the FLE of the diffusion map from Figure 1H (E) or on a zoom-in to the Lepr-MSC cluster in Figure 2A. (F) Distributions of expression levels (TP10K, y axis, censored scale) for select marker genes across the 4 subclusters. Stars for significant differential expression of select genes.

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