Extended Data Table 2. Oligonucleotide primers and siRNAs used in the study.
| Primer (qRT-PCR) | Gene | Sequence (5’-3’) |
|---|---|---|
| IFN-β forward | IFNB1 | ATGACCAACAAGTGTCTCCTCC |
| IFN-β reverse | IFNB1 | GCTCATGGAAAGAGCTGTAGTG |
| L-mRNA EMCV fwd | L-mRNA EMCV | GCGCACTCTCTCACTTTTGA |
| L-mRNA EMCV rev | L-mRNA EMCV | TCGAAAACGACTTCCATGTCT |
| β-actin mRNA forward | ACTB | CTGTGGCATCCACGAAACTA |
| β-actin mRNA reverse | ACTB | AGTACTTGCGCTCAGGAGGA |
| COX1 forward | MT-CO1 | ACGTTGTAGCCCACTTCCAC |
| COX1 reverse | MT-CO1 | TGGCGTAGGTTTGGTCTAGG |
| ND5 forward | MT-ND5 | TCGAAACCGCAAACATATCA |
| ND5 reverse | MT-ND5 | CAGGCGTTTAATGGGGTTTA |
| ND6 forward | MT-ND6 | CCAATAGGATCCTCCCGAAT |
| ND6 reverse | MT-ND6 | AGGTAGGATTGGTGCTGTGG |
| CYB forward | MT-CYB | AGACAGTCCCACCCTCACAC |
| CYB reverse | MT-CYB | GGTGATTCCTAGGGGGTTGT |
| ms Ifit1 forward | Ifit1 | CAAGGCAGGTTTCTGAGGAG |
| ms Ifit1 reverse | Ifit1 | GACCTGGTCACCATCAGCAT |
| ms Gapdh forward | Gapdh | GACTTCAACAGCAACTCCCAC |
| ms Gapdh reverse | Gapdh | TCCACCACCCTGTTGCTGTA |
| siRNA | Gene (alternative name) | List of the StealthRNA oligos |
| siPNPase | PNPT1 (PNPase) | HSS131758 |
| siSUV3 | SUPV3L1 (SUV3) | HSS110378 |
| siMRPP1 | TRMT10C (MRPP1) | HSS123550 |
| siMDA5 | IFIH1 (MDA5) | HSS127414 |
| siTLR3 | TLR3 | HSS110815 |
| siDDX28 | DDX28 | HSS125053 |
| siEXOG | EXOG | HSS115058 |
| siMGME1 | MGME1 | HSS132389 |
| si FEN1 | FEN1 | HSS103629 |
| siRNA | Gene | siRNA Forward Strand |
| siCntrl | Luciferase | GAUUAUGUCCGGUUAUGUAUU |
| siRIG-I | DDX58 (RIG-I) | ACGGAUUAGCGACAAAUUUAA |
| siADAR1 | ADAR (ADAR1) | sc-37657 |
| ON-TARGETplus Human BAK | BAK | L-003305-00-0005 |
| ON-TARGETplus Human BAX | BAX | L -003308-01-0005 |
| ON-TARGETplus Human MAVS | MAVS | L-024237-00-0005 |
| ON-TARGETplus Human SUV3 * | SUPV3L1 | L-017841-01-0020 |
| ON-TARGETplus Human PNPase * | PNPT1 | L-019454-01-0020 |