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. 2019 Jun 15;6:77. doi: 10.1038/s41438-019-0159-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The sgRNA directs the SpCas9 protein to bind genomic DNA through a 20-nucleotide sequence and further guides it to introduce a DSB. This DSB causes random mutations when repaired by the error-prone NHEJ pathway or precise gene modification when repaired by the error-free HDR pathway. CRISPR, clustered regularly interspaced short palindromic repeat; Cas, CRISPR-associated; DSB, double-strand break; HDR, homology-directed repair; NHEJ, non-homologous end-joining; sgRNA, single-guide RNA