Fig. 3.

RCB mediates PHY signaling. a Representative images of 4-d-old Col-0, phyB-9, rcb-10, PBG, and rcb-1/PBG seedlings grown in 0.5 μmol m⁻² s⁻¹ R light. b R light fluence response curves for Col-0, phyB-9, rcb-10, PBG, and rcb-1/PBG measured by growing seedlings for 4 days in darkness or a series of intensities of R light. Hypocotyl length in R light was calculated relative to the value of the corresponding lines in the dark. Error bars represent the SD of three biological replicates. c Representative images of 4-d-old Col-0, phyA-211, rcb-10, PBG, and rcb-1/PBG seedlings grown in 1 μmol m⁻² s⁻¹ FR light. d FR light fluence response curves for Col-0, phyA-211, rcb-10, PBG, and rcb-1/PBG measured by growing seedlings for 4 days in darkness or a series of intensities of FR light. Hypocotyl length in FR light was calculated relative to the value of the corresponding lines in the dark. Error bars represent SD of three biological replicates. e Representative images of 4-d-old Col-0, rcb-10, phyB-9, and rcb-10/phyB-9 seedlings grown in 10 μmol m⁻² s⁻¹ R light. f, Box-and-whisker plots showing hypocotyl measurements of seedlings in (e). g Representative images of 4-d-old Col-0, rcb-10, phyA-211, and rcb-10/phyA-211 seedlings grown in 10 μmol m⁻² s⁻¹ FR light. h Box-and-whisker plots showing hypocotyl measurements of seedlings in (g). i Representative images of 4-d-old YHB, rcb-1/YHB, and PBG seedlings grown in darkness. j Box-and-whisker plots showing hypocotyl measurements of seedlings in (i). k Representative images of 4-d-old Col-0, rcb-10, hmr-5, and rcb-10/hmr-5, and PBG seedlings grown in 10 μmol m⁻² s⁻¹ R light. l Box-and-whisker plots showing hypocotyl measurements of seedlings in (k). For the box-and-whisker plots in (f), (h), (j), and (l), the boxes represent from the 25th to the 75th percentiles, and the bars equal the median values. Different letters denote statistically significant differences in hypocotyl length (ANOVA, Tukey’s HSD, p ≤ 0.001). The source data underlying the hypocotyl measurements in (b), (d), (f), (h), (j), and (l) are provided in the Source Data file