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. 2019 Jun 14;10:2629. doi: 10.1038/s41467-019-10518-0

Fig. 5.

Fig. 5

PIFs repress the assembly and activity of the PEP in the dark. a qRT-PCR results showing the transcript levels of representative PEP- and NEP-dependent genes in 2–4-day-old dark-grown Col-0 and pifq seedlings. The transcript levels were calculated relative to those of PP2A. Fold changes are shown only for the pifq samples exhibiting greater than twofold changes compared with the values of the corresponding Col-0 samples (***p ≤ 0.001). Error bars represent SD of three biological replicates. b Immunoblots showing the level of the PEP complex (blue-native PAGE) as well as the total amount of rpoB and HMR proteins (SDS-PAGE) in 2–4-day-old dark-grown Col-0 and pifq seedlings using antibodies against rpoB or HMR. RPN6 was used as a loading control. The source data underlying the qRT-PCR analysis in (a) and the immunoblots in (b) are provided in the Source Data file