Table 2.
Test panel of compounds for single sensillum recordings from I. typographus OSNs
| Compound | Chemical source Purity % |
Substance origin | Responses in OSN classes1 | |||||
|---|---|---|---|---|---|---|---|---|
| Pcn | tMTol | pC# | cV# | My# | Sty | |||
| Hydrocarbons | ||||||||
| Styrene * | Sigma; >99 | Host | •••• 2 | |||||
| (+)-α-Pinene | Janssens Chim; 98 | Host | •• | (••) | ||||
| (–)-β-Pinene * | Fluka; 92 | Host | ••• | |||||
| Myrcene | Fluka; 95 | Host | (••••) | •••• | ||||
| (+)-3-Carene | Aldrich; 93 | Host | ••• | |||||
| para-Cymene | Acros; >99 | Host | •••• | |||||
| (±)-Limonene | Fluka; 98 | Host | ••• | |||||
| γ-Terpinene | Fluka; 99 | Host | •••• | |||||
| Terpinolene | Fluka; 97 | Host | (•) | (•••) | ||||
| Alcohols | ||||||||
| (+)-Borneol | Sigma 98 | Host | (•) | (•••) | (•) | |||
| 1-Hexanol | Fluka; >99 | NHV | ||||||
| 2-Methyl-3-buten-2-ol | Aldrich; >97 | Ph | ||||||
| E-Myrcenol | SciTech Ltd,; 95 | Ph | ||||||
| (±)-Ipsenol | Bedoukian; 95 | Ph | (•) | |||||
| (±)-Ipsdienol | Bedoukian; 95 | Ph | ||||||
| (±)-3-Octanol | Acros; >99 | NHV/Fungi | ••• | |||||
| 2-Phenylethanol | VWR Int.;>99 | Ph | (•) | (•) | (•) | (•) | •• | |
| (±)-4-Terpineol | Fluka; >99 | Host | (•) | ••• | (•) | (•••) | ||
| (±)-α-Terpineol | Aldrich; 98 | Host | •• | |||||
| (+)-trans-4-Thujanol | Aldrich; >98 | Host | (•) | (•••••) | ||||
| (–)-cis-Verbenol | Borregaard; 95 | Ph | •••• | |||||
| Ketones | ||||||||
| (±)-Camphor | Fluka; >95 | Host | •••• | (•••) | ||||
| (±)-Carvone | Acros; >98 | Host | ••• | (•) | ||||
| Pinocarvone * | Synergy C.; 51a | Host | (•••••) | |||||
| (–)-Verbenone | Fluka; >99 | Host | ••• | |||||
| (±)-α-Thujone | Sigma; >97 | Conifer | (•) | (•) | ||||
| Other oxygenated | ||||||||
| (+)-exo-Brevicomin | W.F.b; 99 | Ph | ||||||
| (±)-Chalcogran | Celamerck; 93 | Ph | (•) | |||||
| (±)-trans-Conophthorin | W.F.; 95 | NHV/Fungi | (••••) | (•) | ||||
| 1,8-Cineole | Aldrich; >99 | Host | (•) | (•) | ||||
| 4-Allylanisole | Acros; 98 | Host | (•••) | ••• | ||||
| Nonanal | Fluka; >95 | Host | ||||||
| No. of cells found | ||||||||
| Male | 5 | 5 | 7 | 2 | 1 | 2 | ||
| Female | 9 | 2 | 0 | 0 | 0 | 0 | ||
| Total | 14 | 7 | 7 | 2 | 1 | 2 | ||
1)Olfactory sensory neuron (OSN) classes are defined according their primary responses. Pcn: Pinocarvone, tMTol: tert. monoterpenols, pC: p-Cymene, cV: cis-Verbenol, My: Myrcene, Sty: Styrene
2)responses: • = 30 – 50 Hz, •• = 51 – 75 Hz, ••• = 76 – 100 Hz, •••• = 101 – 150 Hz, ••••• >150 Hz. Responses in parentheses were found only occasionally or tested few times. #) OSN – class according to Andersson et al. (2009)
aConcentrated extract kindly provided by Synergy Semiochemicals Corp., Burnaby, Canada
bWittko Francke, University of Hamburg, Germany *) compounds that were added to the panel late in the study