SDS–PAGE protein profiles of the 19 Blastocystis isolates separated in 12% polyacrylamide gel and stained by Coomassie blue stain. Lane M, broad range molecular weight standard. Lanes 1–4 in a, lanes 3–5 in b: represent isolates from GI (CRC group). Lanes 5–7 in a, lanes 1–2 in b, and lane 5 in c: isolates from GII (Non-CRC symptomatic group). Lane 8 in a, lane 6 in b and lanes: 1–4 in c: isolates from the GIII (Non-CRC asymptomatic group)