Table 1.
Primer sequences for real-time PCR
| Gene | Sequence (5' → 3') | Length | Tm | GC% |
|---|---|---|---|---|
| AGER | F: ACAGAAACCGGTGATGAAGGA | 21 | 59.3 | 47 |
| R: TCTCCTCGAGTCTGGGTTG | 19 | 58.1 | 57 | |
| GLO-1 | F: TGACGAGACGCAGAGTTACC | 20 | 59.4 | 55 |
| R: GATCTTGAACGAACGCCAGAC | 21 | 59.6 | 52 | |
| AR | F: AGTAGCTGAGGAGTTTCTTCG | 21 | 57.1 | 47 |
| R: CATAGGACTGGAGTTCTAAGCA | 22 | 56.9 | 45 | |
| OGT | F: AGCTCTTCGTCTGTGTCCTA | 20 | 57.8 | 50 |
| R: CAAACTCTGGGAAGACCTCTA | 21 | 56.3 | 47 | |
| GFAT | F: TTGATTCTGATTGCTTGTGGC | 21 | 57.4 | 43 |
| R: ACAGTAGCGAAGACCCATCA | 20 | 58.4 | 50 | |
| GAPDH | F: CATTCTTCCACCTTTGATGCTG | 22 | 57.9 | 46 |
| R: TGGTCCAGGGTTTCTTACTCC | 21 | 58.9 | 52 |
AGER, advanced glycation end products receptor; GLO-1, glyoxalase-1; AR, aldose reductase; OGT, O-GlcNAc transferase; GFAT, glutamine: fructose-6-phosphate aminotransferase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Tm, primer melting temperature; GC%, primer guanine-cytosine content; F, forward; R, reverse