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. 2019 Jun 14;9:8631. doi: 10.1038/s41598-019-45003-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of sesamin in Pro35S:CYP81Q1 and Pro35S:Steroleosin B/Pro35S:CYP81Q1 Arabidopsis plants. (a) The biosynthetic reaction of CYP81Q1. (b) Expression of CYP81Q1 in Pro35S:CYP81Q1 Arabidopsis plants. The CYP81Q1 level was normalized by the value of ACTIN2. Error bars indicate the standard variation (n = 3). (c) The florescence due to sesamin from the authentic standard, and the extracts of wild-type and Pro35S:CYP81Q1 Arabidopsis plants in the UPLC analysis. The overlapped peak with the same retention time was detected in the sample of Pro35S:CYP81Q1 Arabidopsis plants with the addition of the authentic standard (Pro35S:CYP81Q1 + authentic standard). (d) Quantification of sesamin in Pro35S:CYP81Q1 and Pro35S:Steroleosin B/Pro35S:CYP81Q1 plants. Mean values ± standard variations were obtained from four technical replicates.