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. 2019 Jun 14;9:8632. doi: 10.1038/s41598-019-45058-6

Figure 1.

Figure 1

Zebrafish mH2A expression pattern during early embryo development. Wild type zebrafish embryos were collected at different developmental stages. (A) Quantitative RT-PCR was performed for mH2A1, mH2A2 and two genes characteristic of undifferentiated stage (OCT4 and NANOG). Mean values (n = 3) ± SEM are plotted. Values indicate relative expression of the specific gene normalized to GAPDH/TUBULIN. (B) Whole-mount RNA in situ hybridization using mH2A1 and mH2A2 specific probes showing different temporal and spatial gene expression pattern (n = 50–60 embryos/probe with identical labeling).