Table 2.
Summary of functional analysis of RNA-seq relative expression data.
| IPA® analysis summary of RNA-seq data | |||||
|---|---|---|---|---|---|
| Top Canonical Pathways | Molecular and Cellular Functions | ||||
| mH2A1.1–24 hpf vs mH2A1.1–75%epiboly | mH2A1.1–24 hpf vs mH2A1.1–75%epiboly | ||||
| Name | p-value | overlap | Name | p-value | N° of molecules |
| Sirtuin Signaling Pathway | 1,57E-19 | 29,5% 86/292 | Cell Death and Survival | 4,71E-07–4,65E-51 | 909 |
| Oxidative Phosphorylation | 1,38E-18 | 43,1% 47/109 | Gene Expression | 6,73E-08–2,10E-35 | 622 |
| Mitochondrial Dysfunction | 2,35E-17 | 34,5% 59/171 | RNA Post-Transcriptional Modification | 3,10E-09–3,51E-25 | 114 |
| EIF2 Signaling | 2,51E-12 | 26,9% 61/227 | Cell Cycle | 3,19E-07–4,66E-25 | 427 |
| RhoGDI Signaling | 3,76E-10 | 27,1% 48/177 | Cellular Development | 3,27E-07–1,66E-24 | 788 |
| mH2A1.1–75%epi vs mH2A2–75% epiboly | mH2A1.1–75%epiboly vs mH2A2–75% epiboly | ||||
| FXR/RXR Activation | 1,96E-05 | 4,8% 6/126 | Molecular Transport | 2,78E-03–2,95E-10 | 45 |
| Unfolded protein response | 1,07E-04 | 7,1% 4/56 | Lipid Metabolism | 2,48E-03–3,99E-08 | 29 |
| Glucocorticoid Receptor Signaling | 1,40E-04 | 2,3% 8/345 | Small Molecule Biochemistry | 2,48E-03–3,99E-08 | 41 |
| Glycolysis I | 1,98E-04 | 11,5% 3/26 | Energy Production | 1,38E-03–6,92E-07 | 11 |
| Gluconeogenesis I | 1,98E-04 | 11,5% 3/26 | Carbohydrate Metabolism | 2,78E-03–9,03E-06 | 20 |
| mH2A2–24 hpf vs mH2A1–24 hpf | mH2A2–24 hpf vs mH2A1–24 hpf | ||||
| Axonal Guidance Signaling | 2,85E-13 | 18,2% 83/457 | Cell Death and Survival | 1,88E-12–5,85E-38 | 676 |
| Mitochondrial Dysfunction | 2,23E-10 | 23,4% 40/171 | Cellular Movement | 7,86E-09–5,35E-35 | 488 |
| Sirtuin Signaling Pathway | 2,60E-10 | 19,2% 56/292 | Cellular Assembly and Organization | 2,57E-09–7,77E-28 | 408 |
| Oxidative Phosphorylation | 6,27E-10 | 27,5% 30/109 | Cellular Function and Maintenance | 1,41E-09–7,77E-28 | 522 |
| Signaling by Rho Family GTPases | 6,95E-10 | 19,8% 50/252 | Gene Expression | 1,14E-20–1,73E-25 | 422 |
| mH2A2–24 hpf vs mH2A2–75% epiboly | mH2A2–24 hpf vs mH2A2–75% epiboly | ||||
| EIF2 Signaling | 3,72E-31 | 49,3% 112/227 | Gene Expression | 6,89E-12–7,50E-58 | 971 |
| Axonal Guidance Signaling | 6,90E-25 | 35,9% 164/457 | Cell Death and Survival | 2,41E-12–8,16E-57 | 1315 |
| Regulation of eIF4 and p70S6K Signaling | 1,55E-16 | 43,6% 71/163 | Cellular Assembly and Organization | 1,49E-11–5,68E-35 | 749 |
| Sirtuin Signaling Pathway | 4,44E-15 | 34,9% 102/292 | Cellular Function and Maintenance | 1,91E-13–5,68E-35 | 952 |
| Molecular Mechanisms of Cancer | 3,93E-14 | 31,5% 124/394 | Cellular Movement | 4,74E-13–1,14E-33 | 88 |
Functional analysis of differentially expressed genes between cells expressing mH2A1 and mH2A2 at different developmental stages (75% epiboly and 24 hpf) using Ingenuity software analysis. Only the top five represented categories are shown.