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. 2019 Jun 14;18:111. doi: 10.1186/s12934-019-1159-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Optimization of the upstream region of the P3510 promoter. a the upstream regions of the P3510 promoter were changed to corresponding sequence. The nucleotides in bold italic indicate mutated sequences. b The BgaB expression level under the control of P3510 derivatives. c SDS-PAGE analysis of the BgaB expression. Equal amounts (30 μg) of total protein were loaded into each lane. The band corresponding to BgaB was marked. d Comparative analysis of the transcription levels of two promoters at different time points. The transcription level of P_ylb promoter at the time point 4 h was set as 1. The reference gene was 16 s rRNA. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations