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. 2019 Jun 14;18:111. doi: 10.1186/s12934-019-1159-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Intracellular expression of sfGFP. a The fluorescence imagines of strains WBGFP (P_ylb) and WBSGFP (NBP3510). Strains were cultured for 16 h in LB medium and imagines were taken. b The fluorescence intensity controlled by different promoters. c The accumulative sfGFP protein in different strains. M, Marker. Lane 1, Strain WB800. Lane 2, Strain WBGFP. Lane 3, Strain WBSGFP. Equal amounts (30 μg) of total protein were loaded into each lane. The band corresponding to sfGFP was marked. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations