Fig. 5.

Overproduction of Methyl Parathion Hydrolase (MPH) and Chlorothalonil hydrolytic dehalogenase (Chd) using the promoter NBP3510. a The activities of MPH in the supernatant (solid rectangle) and cell density (empty circle) were determined at different times. b The expression of MPH was analyzed by SDS-PAGE. Equal amounts (20 μL) of culture supernatant were loaded into each lane. c The activities of Chd in the supernatant (solid rectangle) and cell density (empty circle) were determined at different times. d The expression of Chd was analyzed by SDS-PAGE. Equal amounts (10 μL) of culture supernatant were loaded into each lane. The bands indicating to the target proteins were marked. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations. CK represents the extracellular protein of strain WB800