Table 1.

Strains and plasmids used in this study

Strain or plasmid	Characteristicsa	Source or referenceb
Plasmids
pAX01	PxylA, Apr, Emr, lacA	[15]
pYBGB	pAX01 containing Pylb-bgaB cassette	This work
pLJ-2	CmR; E. coli-Bacillus shuttle vector	[19]
pUS20	Spcr, unstable in B. subtilis, E. coli-Bacillus shuttle vector	[71]
Strains
E. coli Top10	F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK endA1 nupG	TransGene Biotech (Beijing, China)
B. subtilis
168	trpC2	BGSC 1A1
WB800	(168) ΔnprE, Δ aprA, Δepr, Δ bpr, Δmpr, ΔnprB, Δvpr, ΔwprA	[72]
WBEmBgaB	Strain WB800 derivate, lacA::Pylb-bgaB, Emr	This work
WBBgaB	Strain WB800 derivate, lacA::Pylb-bgaB	This work
BS43	Strain WB800 derivate, lacA::P43-bgaB	This work
BSxylA	Strain WB800 derivate, lacA::PxylA-bgaB	This work
BSsrfA	Strain WB800 derivate, lacA::PsrfA-bgaB	This work
35BgaB	Strain WB800 derivate, lacA::P35-bgaB	This work
10BgaB	Strain WB800 derivate, lacA::P10-bgaB	This work
3510BgaB	Strain WB800 derivate, lacA::P3510-bgaB	This work
351016BgaB	Strain WB800 derivate, lacA::P351016-bgaB	This work
351022BgaB	Strain WB800 derivate, lacA::P351022-bgaB	This work
OBgaB	Strain WB800 derivate, lacA::OP3510-bgaB	This work
JBgaB	Strain WB800 derivate, lacA::JP3510-bgaB	This work
DBgaB	Strain WB800 derivate, lacA::DP3510-bgaB	This work
BBgaB	Strain WB800 derivate, lacA::BP3510-bgaB	This work
WBSBgaB	Strain WB800 derivate, lacA::NBP3510-bgaB	This work
WBGFP	Strain WB800 derivate, lacA:: Pylb -sfGFP	This work
WBSGFP	Strain WB800 derivate, lacA::NBP3510-bgaB	This work
WBSMPH	Strain WB800 derivate, lacA::NBP3510-mpd	This work
WBSChd	Strain WB800 derivate, lacA::NBP3510-chd	This work

^aAp^R: ampicillin resistance; Cm^R: chloramphenicol resistance; Em^R: erythromycin resistance; Spc^r: spectinomycin resistance

^bBGSC: Bacillus Genetic Stock Center