Fig. 7.

a ALT1 allele frequency in hiPSC, 520d/progenitor hMSC, or hMSC. hiPSC had non-negligible number of mutated sites, but the total number of mutations were reduced and some of them were converted to wild type during the differentiation process after 520d-5p transfection. b Circle graph showing allelic status induced by 520d-5p transfection. The decrease of ALT1 allele frequency was observed in 60.3% (301,733/500,189) of mutated sites. 11.1% (58,519/500,189) gained the new mutated regions. c. Scatter plot showing alterations of nucleotides during the differentiation towards hMSC status in the increased mutations (585,199 sites in 2)). Ratio of allele frequency 520d-5p-transfected hMSC progenitor cells / normal hMSC) was estimated. A large parts of alteration of increased sites in progenitor cells seemed to be an alteration accompanied by the differentiation process towards hMSC status according to the standardization to nucleotides status observed in hMSC