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. 2019 Jun 15;14:58. doi: 10.1186/s13000-019-0835-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Pictures show the negligibly small effect of the preparation process for scanning on the final pathologic routine: On-site scanning procedure did not interfere with the subsequent control immunohistochemical staining process. Immunohistochemical stains of two cases. Case 22: invasive ductal (NST) carcinoma. a and b: Estrogen receptor (ER) stain, low expression. a HE stain, regular formalin fixation. B HE stain, formalin fixation after confocal microscopy. c and d: Ki-67 proliferation index. c HE stain, regular formalin fixation. d HE stain, formalin fixation after confocal microscopy. Case 23: high grade DCIS. e and f: ER stain, high expression. e HE stain, regular formalin fixation. f HE stain, formalin fixation after confocal microscopy. g and h: Basal cytokeratins (CK5/6). g HE stain, regular formalin fixation. h HE stain, formalin fixation after confocal microscopy