Table 1.
Classification and counts for the sequencing results
| Sample | Read pairs | Effective reads | Unique Vβ | Unique Jβ | Unique VDJ | Unique CDR3aa |
|---|---|---|---|---|---|---|
| Control | 5227607 | 4297968 | 62 | 14 | 1499 | 17789 |
| Cytokines | 5110352 | 3254496 | 64 | 14 | 1448 | 13828 |
| WT1 | 6591869 | 4968087 | 62 | 14 | 1437 | 14472 |
| B3A2 | 5854623 | 3542234 | 64 | 14 | 1393 | 11747 |
Control: the untreated group; Cytokines: cells cultured with IL-2 and Il-7 in vitro for 21 days; WT1: cells treated with WT1 peptide (RMFPNAPYL, AA 126–134) and cultured with IL-2 and IL-7 in vitro for 21 days; B3A2: cells treated with 6 different BCR-ABL peptides and cultured with IL-2 and IL-7 in vitro for 21 days; An effective read is a read possessing the full CDR3 structure; Unique Vβ: types of Vβ gene usage; Unique Jβ: types of Jβ gene usage; Unique VDJ: unique combinations of V/D/J genes; Unique CDR3aa: unique CDR3 amino acid sequences