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. 2019 Jun 14;19:585. doi: 10.1186/s12885-019-5818-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of PRAF2 on proliferation and cell cycle in ESCC cells. a Relative PRAF2 mRNA expression was determined using qPCR. b PRAF2 protein was detected by western blot in TE1 cells with PRAF2 silencing. The relative density of PRAF2 was quantified and normalized to GAPDH. c CCK-8 assay was performed to determine the ability of cell proliferation. c Colony formation assay was performed to determine the clonogenic capacity of TE1 cells. d The bar chart represented the number of colonies. e The cell cycle distribution was determined using flow cytometry. f The histogram of quantitative analysis in different mitotic phases. Values represented the mean ± SD data in triplicate. **P < 0.01