. 2019 Jun 12;7:e6723. doi: 10.7717/peerj.6723

Table 4. List of sex-related protein; protein with loading value greater than 0.65 are bold typed.

Protein	Spot N	Statistically significant RATIO	Fold-change	Loading value
Spots detected only in Male comparing silkworm reared on the same diet
Putative cuticle protein	31	M_L/F_L	Only in M_L	0.218
Tubulin beta chain	39	M_L/F_L	Only in M_L	0.064
Masquerade-like serine proteinase homolog	39	M_L/F_L	Only in M_L	0.064
Hemolin	95	M_L/F_L	Only in M_L	0.544
Heat shock protein 70-5	100	M_A/F_A	Only in M_A	0.571
Heat shock cognate protein	100	M_A/F_A	Only in M_A	0.571
Antichymotrypsin	126	M_A/F_A	Only in M_A	0.553
Mitochondrial aldehyde dehydrogenase	135	M_A/F_A	Only in M_A	0.497
Actin-depolymerizing factor 1	152	M_A/F_A	Only in M_A	0.474
Arginine kinase	167	M_A/F_A	Only in MA	0.219
Arginine kinase	167	M_L/F_L	Only in ML	0.219
SP1	237	M_A/F_A	Only in M_A	−0.159
Transferrin	239	M_A/F_A	Only in M_A	−0.138
ML-domain containing secreted protein	247	M_A/F_A	Only in M_A	0.336
acyl-CoA dh isoform X2	263	M_A/F_A	Only in MA	0.257
Imaginal disk growth factor	266	M_L/F_L	Only in ML	0.674
Bifunctional purine biosynthesis protein	275	M_A/F_A	Only in M_A	0.545
Bifunctional purine biosynthesis protein	275	M_L/F_L	Only in ML	0.545
Pyruvate kinase	276	M_A/F_A	Only in M_A	0.716
Catalase
Glucose-6P-1 dehydrogenase		M_L/F_L	Only in ML
Transferrin	279	MA/FA	Only in MA	0.534
Spots detected only in Female comparing silkworm reared on the same diet
SP1	81	M_A/F_A	Only in F_A	−0.422
Antichymotrypsin-1	90	M_L/F_L	Only in F_L	0.492
Egg-specific protein	190	M_L/F_L	Only in F_L	−0.531
Egg-specific protein	191	M_L/F_L	Only in F_L	−0.681
Vitellogenin LC	124	M_A/F_A	Only in F_A	−0.536
	124	M_L/F_L	Only in F_L	−0.536
	172	M_A/F_A	Only in F_A	−0.575
	172	M_L/F_L	Only in F_L	−0.575
	173	M_A/F_A	Only in F_A	−0.674
	173	M_L/F_L	Only in F_L	−0.674
	225	M_A/F_A	Only in F_A	−0.663
	225	M_L/F_L	Only in F_L	−0.663
	226	M_A/F_A	Only in F_A	−0.437
Vitellogenin HC	242	M_A/F_A	Only in F_A	−0.631
	242	M_L/F_L	Only in F_L	−0.631
	243	M_L/F_L	Only in F_L	−0.619
	244	M_A/F_A	Only in F_A	−0.571
	244	M_L/F_L	Only in F_L	−0.571
	245	M_L/F_L	Only in F_L	−0.591
	283	M_A/F_A	Only in F_A	−0.494
	283	M_L/F_L	Only in F_L	−0.494
	284	M_A/F_A	Only in F_A	−0.437
	284	M_L/F_L	Only in F_L	−0.437
	286	M_L/F_L	Only in F_L	−0.529
Apolipophorin protein	312	M_L/F_L	Only in F_L	−0.255
SP1	332	M_A/F_A	only in F_A	−0.544
Arylphorin
Spots up-regulated in Male
Putative cuticle protein	10	M_A/F_A	2.96**	0.633
32 kDa apolipoprotein	32	M_A/F_A	1.70**	0.677
Hemolin	95	M_A/F_A	2.34*	0.544
Cellular retinoic acid binding protein	111	M_A/F_A	3.23**	0.729
Mitochondrial aldehyde dehydrogenase	135	M_L/F_L	1.87*	0.497
Glutathione S-transferase sigma	157	M_L/F_L	2.63**	0.100
Phenoloxidase subunit 1	198	M_L/F_L	1.72	−0.081
Ferritin	250	M_A/F_A	2.31**	0.706
Fructose-bisphosphate aldolase	259	M_A/F_A	1.71*	0.767
Fructose-bisphosphate aldolase	259	M_L/F_L	2.04*	0.767
Glutathione S-transferase delta	295	M_L/F_L	2.23*	0.474
ATP synthase α subunit	328	M_L/F_L	1.75**	0.051
Spots up-regulated in Female
Antichymotrypsin-1	67	F_A/M_A	1.73**	0.011
Dynein heavy chain 2	181	F_L/M_L	1.84**	−0.593

Notes:

F_A, female artificial diet; F_L, female mulberry leaves; M_A, male artificial diet; M_L, male mulberry leaves.

P < 0.05.

^**

P < 0.01.