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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to add Starting concentration Final concentration
(1X)
Tris-HCl, pH 7.4 10 mL 1 M 20 mM
NaCl 15 mL 5 M 150 mM
Triton X-100 2.5 mL 20% 0.1 %
BSA 10 g powder 2 %
NaN3 2.5 mL 20 % 0.1 %