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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to add Starting concentration Final concentration
(1X)
K-HEPES, pH 7.6 1 mL 1 M 10 mM
KCl 10 mL 2 M 200 mM
MgCl2 0.1 mL 1 M 0.5 mM
K-EGTA 0.2 mL 0.5 M 0.5 mM
Sucrose 8.57 g solid 250 mM