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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to add Starting concentration Final concentration
(1X)
MMR 2 mL 25 x 1 x
Triton X-100 1.25 mL 20 % 0.5 %
Glycerol 10 mL 100 % 20 %
Formaldehyde 3.65 mL 37 % 2.7 %