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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to add Starting concentration Final concentration
(35X)
ATP 10 μL 200 mM ~67 mM
Creatine phosphate 20 μL 1 M ~667 mM
Creatine phosphokinase 0.8 μL 5 mg/mL 0.13 mg/mL