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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to
add 		Starting concentration Final concentration
Tris-HCl, pH 8.8 0.8 mL 1 M 20 mM
p-phenylenediamine (free base) 0.20 g powder 0.5 %
Glycerol 36 mL 100 % 90 %