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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cold Spring Harb Protoc. 2019 Feb 1;2019(2):pdb.prot097121. doi: 10.1101/pdb.prot097121
Reagent Amount to
add 		Starting concentration Final concentration
MMR 80 μL 25 x 1 x
DAPI 2 μL 1 mg/mL 1 μg/mL
Glycerol 1.2 mL 100 % 60 %
Formaldehyde 0.59 mL 37 % 11 %
H2O 128 uL