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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Hum Cell. 2019 Mar 14;32(3):240–250. doi: 10.1007/s13577-019-00247-3

Figure 5: SLFN12 modulates repetitive mechanical deformation-induced sucrase-isomaltase gene expression in human intestinal epithelial Caco-2 cells.

Figure 5:

Caco-2 cells were plated on collagen-I coated flexible membranes and next day cells were transfected with control siNT1 or siSLFN12 for 48 hours followed by mechanical strain at 10cycle/min for 24 hours. Cells cultured in parallel in the absence of mechanical strain served as controls and SLFN12 and SI gene expression were measured by qPCR. Repetitive mechanical deformation enhances (A) SLFN12 and (B) SI expression in siNT1-treated cells (n = 5, *, p < 0.05). Reduced SLFN12 expression by siSLFN12 attenuated deformation-induced (A) SLFN12 and (B) SI expression (n=5, #, p<0.05). SLFN12 siRNA inhibited basal (A) SLFN12 and (B) basal SI expression (n = 5, *, p < 0.05).