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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Hum Cell. 2019 Mar 14;32(3):240–250. doi: 10.1007/s13577-019-00247-3

Figure 6: Co-stimulatory effects of repetitive mechanical deformation and butyrate on SLFN12 and SI-expression in the absence and presence of siRNA to SLFN12.

Figure 6:

(A) SLFN12 and (B) SI expression analyzed by qTR-PCT in Caco-2 cells transfected with siNT-1 control siRNA or the siRNA-1/siRNA-4 mixture to reduce SLFN12. Forty-eight hours after transfection, these cells were maintained for an additional 24 hours under control conditions (black bars), with cyclic strain (shaded bars from lower left to upper right), 2 mM butyrate (shaded bars from upper right to lower left), or in combination for co-stimulation (cross-hatched bars). Cells not exposed to cyclic strain were cultured under static conditions and cells not treated with butyrate were treated with an equal volume of PBS as a vehicle control (n=4, *p<0.05, siNT static vs either strain or static-NaB or strain-NaB).