Figure 2. CHCHD10S55L mice manifest motor symptoms and skeletal muscle atrophy but no cognitive impairment.
A. Forepaw grip strength expressed as percent of WT average. n=10 mice per genotype (n=5 females, n=5 males). Error bars indicate SD. *p<0.05, **p<0.01, ***p<0.001 by ANOVA with repeated measures, Holm-Sidak correction for multiple comparisons. B. Exercise endurance measured by time on the treadmill expressed as percent of WT average. n=10 mice per genotype. Error bars indicate SD. *p<0.05, **p<0.01, ***p<0.001 by ANOVA with repeated measures, Holm-Sidak correction for multiple comparisons. C. Image of hind limb musculature of CHCHD10S55L heterozygote, WT, and CHCHD10 KO mice at 330 days of age. D. Mice on the pole descent task. CHCHD10S55L mice displayed hindlimb incoordination characterized by poor hindpaw pole grasping (magnified images, arrows). E. Average pole descent time in mice at 330 days of age. n=3 mice per group, 3 trials each mouse. Error bars indicate SEM. *p<0.05, by Student’s t-test. F. and G. Novel object recognition test. Average time spent with the novel object and total exploration time recorded during a 5-min testing period in mice at 250 days of age. n=13 WT (7 males and 6 females) and n=16 CHCHD10S55L mice (8 males and 8 females) mice per group. Error bars indicate SEM. H. and I. Open field test. Average percent time spent in the center field relative to total time, and total distance travelled. n=13 WT (7 males, 6 females) and n=16 CHCHD10S55L (8 males, 8 females) per group. Error bars indicate SEM. **p<0.01 by Student’s t-test.