Figure 4. Histopathological and ultrastructural abnormalities in CHCHD10S55L muscle and heart.
A. and B. H&E staining of myocardium in the apical region of hearts from mice at 330 days of age. The right panel of each image is a magnification of the area indicated by the square in the left panel. In B, arrows indicate cytoplasmic vacuoles. Scale bar = 50 μm. C. and D. Masson’s trichrome stain of myocardium of mice at 330 days of age. The right panel of is a magnification of the area indicated by the square in the left panel. In D, arrows indicate areas of cardiac interstitium with abundant collagen accumulation. Scale bar = 50 μm. E. Electron micrographs of mitochondria in WT, CHCHD10S55L, and CHCHD10 KO cardiac tissue. Arrows highlight abnormal membranous structures in mitochondria. Scale bar = 500 nm. F. Quantification of abnormal heart mitochondria (% of total mitochondria). G and H. H&E staining of quadriceps femoris. The right panel of is a magnification of the area indicated by the square in the left panel. Scale bar = 100 μm. I. Electron micrographs of WT, CHCHD10S55L, and CHCHD10 KO skeletal muscle. Scale bar = 1 μm. J. Quantification of abnormal skeletal muscle mitochondria (% of total mitochondria).