Figure 6. CHCHD10S55L mice have abnormal mitochondria in dopaminergic neurons.
A. TH immunostaining of substantia nigra pars compacta. Bars = 100 μm. B. Average TH+ neurons count in a 200 × 500 μm area encompassing the substantia nigra pars compacta expressed relative to WT. n=4 mice, 4 areas per mouse. n.s., not significantly different by Student’s t-test. C. Electron micrographs of mitochondria in immunogold labeled TH+ neurons of the substantia nigra. Abnormal cristae and vacuolization are visible in CHCHD10S55L TH+ neurons. The right panel of each image is a magnification of the area indicated by the square in the left panel. Scale bar = 1 μm. D. Quantification of abnormal mitochondria in TH+ neurons (% of total mitochondria).