Fig. 3.

Profile of marker peptides released under optimised digestion conditions over 35 h of digestion. The addition of trypsin was static and multiple. Static addition of trypsin was performed only at time 0 h at two E:S ratios—1:50 and 1:100. Multiple additions of trypsin were performed at equidistant intervals of time to a final 1:25 (E:S) ratio—1:50 (E:S) ratio was added at time 0 and time 22 h; 1:100 (E:S) ratio was added at time 0, 6, 12 and 22 h. Error bars represent the standard deviation of 3 replicate measurements. a α S1 CN-YLGYLEQLLR. b α S2 CN-FALPQYLK. c Ova-LYAEER. d Lys-FESNFNTQATNR